RNA

Part:BBa_K4501002:Design

Designed by: Pau Marín Escudero   Group: iGEM22_Barcelona_UB   (2022-09-08)


anti-myc shRNA (1741-1761) in-loop C/D-box


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The structure of the shRNA was validated on Benchling software. The potential gene silencing was evaluated very high (score 13.2 out of 15) by VectorBuilder software.


Source

Designed by ourselves using the VectorBuilder software to design shRNA (https://en.vectorbuilder.com/design) on human MYC gene (NM_002467.6).

References